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Safety Data Sheet (SDS) PE Anti-mouse Tim-2 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse Tim-2 Antibody
1245030 100 µg $260.00       
Clone: F37-2C4
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance in each application.

Mouse Tim-2 transfected cells stained

Mouse Tim-2 transfected cells stained with F37-2C4 PE



Description:

Tim-2 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-2. It is expressed on activated T cells and preferentially on differentiated Th2 cells. Sema4A is the ligand of Tim-2. A blockade of Tim-2/Tim-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. Tim-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.

Other Names: T cell immunoglobulin and mucin domain containing protein-2
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 33 kD
Distribution: Developmentally regulated; highly expressed in embryonic development at the 8-cell stage and blastocyst. Expressed on activated T lymphocytes.
Ligand Receptor: Sema4A, H-ferritin
Antigen References:

1. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
2. Kumanogoh A, et al. 2002. Nature 419:629.
3. Chakravarti S, et al. 2005. J. Exp. Med. 202:437