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Safety Data Sheet (SDS) Biotin Anti-human/mouse CXCR7 Antibody     Product Data Sheet (PDF)    
Biotin Anti-human/mouse CXCR7 Antibody
2255555 25 µg $115.00       
2255560 100 µg $230.00       
Clone: 8F11-M16
Isotype: Mouse IgG2b, κ
Reactivity: Human, mouse
Immunogen: Human CXCR7 transfectant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested
ICFC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: Intracellular staining for flow cytometry.2

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Soehnlein O, et al. 2013. EMBO Mol. Med. 5:471. (FC) PubMed
2. Saad ST, et al. 2014. PLoS One 9(1). (ICFC) PubMed
3. Balabanian K, et al. 2012. J. Transl. Med. 10:251. (FC) PubMed

CXCR7 transfected L1.2 cells were

CXCR7 transfected L1.2 cells were stained with biotinylated CXCR7 (clone 8F11-M16) (filled histogram) or biotinylated mouse IgG2b isotype control (open histogram), followed by Sav-PE.



Description:

CXCR7, also known as RDC1, belongs to a subgroup of C-X-C chemokine receptors, which are part of a large protein family of G protein-coupled receptors (GPCR).  CXCR7 binds with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC, which regulates the trafficking and activation of leukocytes. It is also a co-receptor for the entry of HIV-1. Binding of ligand to CXCR7 induces proliferation and migration of immature neurons, glia and their precursors.  CXCR7 expression occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells.  Surface expression of CXCR7 was also reported to be on tumor cells, activated endothelial cells, fetal liver cells, and other cell types.

Other Names: Chemokine (C-X-C motif) receptor 7, RDC1, CMKOR1, GPR159
Structure: G Protein-Coupled Receptor super family
Distribution: Monocytes, B cells, T cells and mature dendritic cells
Ligand Receptor: CXCL12/SDF-1 and CXCL11/I-TAC
Regulation: Regulates the trafficking and activation of leukocytes
Cellular Sources: On a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells
Antigen References:

1. Burns JM, et al. 2006. J. Exp. Med. 203:2201.
2. Infantino S, et al. 2006. J. Immunol. 176:2197.