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Safety Data Sheet (SDS) Purified Anti-mouse TCR Gamma/delta Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse TCR γ/δ Antibody
1137510 500 µg $190.00       
Clone: UC7-13D5
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation1,2,4 and in vivo depletion3-5.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Bluestone JA, et al. 1991. Immunol. Rev. 120:5. (IP Costim)
2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology p. 3.12.1 - 3.12.14. (Costim)
3. Hiromatsu K, et al. 1992. J. Exp. Med. 175:49. (Deplete)
4. Dieli F, et al. 1997. Eur. J. Immunol. 27:206. (Costim)
5. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
5. Pollinger B, et al. 2011. J. Immunol. 186:2602. PubMed
6. Dong P, et al. 2014. PLoS Pathog. 9:112964. PubMed

C57BL/6 splenocytes stained with 17A2

C57BL/6 splenocytes stained with 17A2 (CD3) APC and purified UC7-13D5, followed by anti-Armenian hamster IgG PE



Description:

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. Immobilized UC7-13D5 antibody has been reported to activate TCR-γ/δ-bearing T cells in vitro, and to deplete peripheral TCR-γ/δ-bearing T cells in vivo.

Other Names: TCR-γ/δ, γ/δ TCR
Structure: Ig superfamily, associates with CD3 complex
Distribution: T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum
Function: Antigen recognition
Ligand Receptor: Some bacterial or tumor antigens bound MHC class I
Antigen References:

1. Skarstein K, et al. 1995. Immunology 81:497.
2. Harrison LC, et al. 1996. J. Exp. Med. 184:2167.
3. Wildner G, et al. 1996. Eur. J. Immunol. 26:2140.
4. Brandes M, et al. 2005. Science 309:264.