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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD199 CCR9 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD199 (CCR9) Antibody
1248545 25 µg $120.00       
1248550 100 µg $290.00       
Clone: 9B1
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

C57BL/6 thymocytes stained with 9B1

C57BL/6 thymocytes stained with 9B1 Alexa Fluor® 647


CCR9 is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in gut-associated immune response. Mouse CCR9 is highly expressed on different stages of thymocytes and upregulated on CD4+CD8+ cells. It is expressed to a much lower level on spleen and lymph node cells. Expression of CCR9 on γ/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory.

Other Names: GPR 9-6, CDw199, Chemokine Receptor 9
Structure: A membrane of G-protein coupled receptors and contains seven transmembrain domains. Mouse CCR9 has above 80% amino acid sequence homology with human counterpart.
Distribution: CD4+CD8+ thymocytes, γ/δ T cells
Ligand Receptor: CCL25
Interaction: CCL25
Antigen References:

1. Zaballos A, et al. 1999. J. Immunol. 162:5671
2. Wurbel MA, et al. 2007. J. Immunol. 178:7598
3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73

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