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Safety Data Sheet (SDS) PE Anti-mouse CD196 CCR6 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD196 (CCR6) Antibody
1249015 25 µg $115.00       
1249020 100 µg $275.00       
Clone: 29-2L17
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Mony JT, et al. 2014. Mult Scler. PubMed
2. Shibata K, et al. 2014. J. Immunol. 192:2210. PubMed

C57BL/6 splenocytes stained RA3-6B2 (B220)

C57BL/6 splenocytes stained RA3-6B2 (B220) FITC and 29-2L17 PE



Description:

CCR6 is a G-protein linked chemokine receptor which binds the chemokine CCL20/MIP-3a. It is expressed on B lymphocytes and certain subsets of dendritic cells and T cells. CCR6 is reported to be involved in mucosal immune response and lymphocyte migration and homeostasis.

Other Names: CD196, BN-1, CKR-L3, DRY6, KY411, STRL22
Structure: G-protein linked, seven transmembrane, domain-spanning chemokine receptor; 367 amino acids long and about 75% homology with human CCR6
Distribution: Expressed on spleen B cells, immature splenic dendritic cells in Peyer's patches, and some T cell subset
Function: Important in regulating mucosal immune response
Ligand Receptor: CCL20/MIP-3a
Antigen References:

1. Rossi D, et al. 1997. J. Immunol. 158:1033.
2. Lukacs N, et al. 2001. J. Exp. Med. 194:551.
3. Cook DN, et al. 2000. Immunity 12:495