The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Additional reported applications (for the relevant formats) include: To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 , is recommended prior to immunofluorescent staining.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Yamashita Y, et al. 1999. J. Immunol. 162:5940. 2. Kouro T, et al. 2002. Blood 100:3672. 3. Maeda K, et al. 2005. Blood 106:879. 4. Diao J, et al. 2004. J. Immunol. 173:1826.
C57BL/6 splenocytes stained with SB/199 APC and CD3 (145-2C11) FITC
CD127 is a 60-90 kD type I transmembrane glycoprotein, also known as IL-7 receptor α chain or IL-7Rα. It forms heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
IL-7 receptor α chain, IL-7Rα
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
T cell and immature B cell proliferation and development
1. Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:9125. 2. He YW and Malek TR. 1998. Crit Rev. Immunol. 18:503. 3. Huster K M,et al. 2004. Proc. Natl. Acad. Sci. USA. 101:5610. 4. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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