The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Lodoen M, et al. 2003. J. Exp. Med. 197:1245. This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
YAC-1 cell line stained with CX1 PE
RAE-1 gamma is one of the five member RAE-family.Together with H60 they were identified as ligands for the mouse NKG2D molecules.Expression of RAE-1 is low or absent in normal adult tissue, but can be upreulgated by retinoid acid and constitutively expressed on some tumors.This antibody has weak crossreativity with RAE-1 alpha and RAE-1 beta.
Retinoid acid early inducible gamma
RAE is a family of GPI-anchord surface protein consisting of alpha, beta, gamma, delta and epsilon. They share strong homology within the family and weak homology with MHC class 1 molecule.
Expression of RAE-1 is low or absent on normal, adult tissue, but consititutively expressed on some tumors and can be induced by retinoid acid.
As ligands for the mouse activating NKG2D expressed by NK cells
1. Cerwenka A, et al. 2000. Immnity. 12:721 2. Lodoen M, et al. 2003 J. Exp. Med. 197:1245 3. Diefenbach A, et al. 2001 Nature 413:165
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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