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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse RAE-1gamma Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse RAE-1γ Antibody
1250550 100 µg $190.00       
Clone: CX1
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse RAE-1 gamma transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Lodoen M, et al. 2003. J. Exp. Med. 197:1245.
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

YAC-1 cells stained with CX1

YAC-1 cells stained with CX1 Alexa Fluor® 647



Description:

RAE-1 gamma is one of the five member RAE-family.Together with H60 they were identified as ligands for the mouse NKG2D molecules.Expression of RAE-1 is low or absent in normal adult tissue, but can be upreulgated by retinoid acid and constitutively expressed on some tumors.This antibody has weak crossreativity with RAE-1 alpha and RAE-1 beta.

Other Names: Retinoid acid early inducible gamma
Structure: RAE is a family of GPI-anchord surface protein consisting of alpha, beta, gamma, delta and epsilon. They share strong homology within the family and weak homology with MHC class 1 molecule.
Distribution: Expression of RAE-1 is low or absent on normal, adult tissue, but consititutively expressed on some tumors and can be induced by retinoid acid.
Function: As ligands for the mouse activating NKG2D expressed by NK cells
Ligand Receptor: NKG2D
Antigen References:

1. Cerwenka A, et al. 2000. Immunity 12:721
2. Lodoen M, et al. 2003 J. Exp. Med. 197:1245
3. Diefenbach A, et al. 2001 Nature 413:165


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.