The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
Additional reported applications (for the relevant formats of this clone) include: in vivo induction of auto-antibody production1 and blockade of dendritic cell Tim-42.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Nakayama M, et al. 2009. Blood. 113:3821. (FA) 2. Yeung MY, et al. 2013. J. Immunol. 191:4447. (Block)
Tim-4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells. Tim-4 binds to Tim-1 to promote T cell proliferation by enhancing cell division and reducing apoptosis. Tim-4 bind to phosphatidylserine through its FG-CC’ binding cleft in the N-terminal IgV domain to facilitate the clearance of apoptotic cells or bodies.
T cell immunoglobulin and mucin domain containing protein-4
Transmembrane protein containing immunoglobulin domain and mucin-like domain
Unlike the other Tims, Tim-4 is only expressed by antigen-presenting cells, but not by T cells.
Tim-4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
Phosphatiylserine is the ligand of Tim-4 and Tim-4 is a ligand of Tim-1.
1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577 2. Miyanishi M, et al. 2007. Nature 450:435 3. Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706
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