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Safety Data Sheet (SDS) PE Anti-mouse Tim-4 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse Tim-4 Antibody
1250025 25 µg $95.00       
1250030 100 µg $260.00       
Clone: RMT4-54
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse TIM4-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats of this clone) include: in vivo induction of auto-antibody production1 and blockade of dendritic cell Tim-42.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Nakayama M, et al. 2009. Blood. 113:3821. (FA)
2. Yeung MY, et al. 2013. J. Immunol. 191:4447. (Block)

BALB/c peritoneal macrophages stained with

BALB/c peritoneal macrophages stained with RMT4-54 PE and M1/70 (CD11b) FITC



Description:

Tim-4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells. Tim-4 binds to Tim-1 to promote T cell proliferation by enhancing cell division and reducing apoptosis. Tim-4 bind to phosphatidylserine through its FG-CC’ binding cleft in the N-terminal IgV domain to facilitate the clearance of apoptotic cells or bodies.

Other Names: T cell immunoglobulin and mucin domain containing protein-4
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain
Distribution: Unlike the other Tims, Tim-4 is only expressed by antigen-presenting cells, but not by T cells.
Function: Tim-4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
Ligand Receptor: Phosphatidylserine, TIM1
Interaction: Phosphatiylserine is the ligand of Tim-4 and Tim-4 is a ligand of Tim-1.
Antigen References:

1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577
2. Miyanishi M, et al. 2007. Nature 450:435
3. Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706