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Safety Data Sheet (SDS) PE Anti-mouse CD314 NKG2D Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD314 (NKG2D) Antibody
1251035 50 µg $95.00       
1251040 200 µg $285.00       
Clone: CX5
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Mouse NKG2D
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

To reduce non-specific binding, preincubate cells with purified anti-mouse CD16/CD32, clone 93 (Cat No 101301/2) is recommended.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ogasawara K, et al. 2003. Immunity 18:41. (Block)
2. Ogasawara K, et al. 2004. Immunity 20:757. (Block)
3. Kong LY, et al. 2010. Clin. Cancer Res. 16:2550. PubMed

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

C57BL/6 splenocytes stained with DX5

C57BL/6 splenocytes stained with DX5 FITC and CX5 PE



Description:

This antibody has been reported to block binding of NKG2D to its ligands, RAE-1 and H60, and inhibition of NKG2D-dependent NK-cell cytotoxicity.

Other Names: CD314
Structure: NKG2D is a lectin-like type II transmembrane protein. It serves as a stimulatory receptor to activate NK cells via the non-covalently associated DAP10 or DAP 12 daptor protein.
Distribution: NK, NKT cells
Ligand Receptor: RAE, H60