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Safety Data Sheet (SDS) Purified Anti-mouse Notch 1 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse Notch 1 Antibody
1253010 500 µg $330.00       
Clone: HMN1-12
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Notch1-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Moriyama Y, et al. 2008. Int. Immunol. 20:763.

Mouse Notch-1 transfected cells stained

Mouse Notch-1 transfected cells stained with purified HMN1-12, followed by anti-Armenian hamster IgG PE



Description:

The Notch receptors are highly conserved from invertebrates to mammals. Notch 1 and Notch 2 exhibit the greatest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences. The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus. This translocation triggers a series of signaling processes. Notch 3 is primarily expressed in adult arterial smooth muscle cells. Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.

Other Names: Neurogenic locus notch homolog protein 1
Structure: Transmembrane receptor. Heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution: Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart.
Function: Regulates broad spectrum of various cell lineage commitment processes, including myogenesis, neurogenesis, gliogenesis, and lymphocyte development.
Ligand Receptor: Jag 1,2; Delta 1,2
Antigen References:

1. Ehebauer ME, et al. 2006. Biochem. J. 392:13.
2. Shimizu K, et al. 2000. Mol. Cell. Biol. 20:18.
3. Tanigaki K, et al. 2007. Nature Immunol. 8:4514.
4. Kraman M, et al. 2005. FASEB J. 19:1311.