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Safety Data Sheet (SDS) Purified Anti-mouse Notch 3 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse Notch 3 Antibody
1252510 500 µg $330.00       
Clone: HMN3-133
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Notch 3-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Moriyama Y, et al. 2008. Int J Immunology 20:763
2. Ghosh S, et al. 2011. J. Biol Chem. 286:22678. PubMed.
3. Romano RA, et al. 2012. Development 139:772. PubMed.



Description:

The Notch receptors are highly conserved from invertebrates to mammals.While Notch1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences.The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus.This translocation triggers a series of signaling process.Notch 3 is primarily expressed in adult arterial smooth muscle cells.Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.

Other Names: Neurogenic locus notch homolog protein 3, Notch-3,CADASIL
Structure: Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution: Notch 3 is primarily expressed in vascular smooth muscle cells, also found in proliferating neuroepithelium.
Function: Critical role in the structural integrity of distal resistance artieries. Mutations in the Notch 3 receptor on vascular smooth muscles cells is associated with an inherited neurodegenerative disease CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopthy)
Ligand Receptor: Jag1,2; Delta 1,2
Antigen References:

1. Ehebauer ME, et al. 2006. Biochem J 392:13
2. Shimizu K, et al. 2000. Mol Cell Biology 20:18
3. Tanigaki K, et al. 2007. Nature Immunol 8:451
4. Bellavia D, et al. 2008. 27:5092
5. Louvi A, et al. Dev Neurosci. 2006. 28:5