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Safety Data Sheet (SDS) APC Anti-mouse DLL4 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse DLL4 Antibody
1254065 25 µg $130.00       
1254070 100 µg $300.00       
Clone: HMD4-1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: CHO cells expressing murine DLL4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Moriyama Y, et al. 2008. Int. Immunol. 20:763.

Mouse Dll4 transfected cells stained

Mouse Dll4 transfected cells stained with HMD4-1 APC



Description:

The Notch receptors and their ligands are highly conserved from invertebrates to mammals. Delta-like 4 (DLL4) is one of four or five Notch ligands identified. The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus. This translocation triggers a series of signaling processes. DLL4 is reported to be essential for the regulation of angiogenesis. In thymus, DLL4 is an essential Notch1 ligand responsible for T cell lineage commitment.

Other Names: DLL 4
Structure: A single-pass transmembrane (TM) protein consisting of a large extracellular N-terminal which includes the DSL domain, followed by a tandem array of EGF-like repeats (ELRs). After the single TM domain is a novel intracellular domain.
Distribution: DLL4 is primarily expressed in arterial blood vessels and sprouting endothelial cells. DLL4 is also expressed in thymic epithelial cells.
Function: The Notch receptors and their ligands are highly conserved signaling molecules throughout development. They regulate cell fate of various cell's development, including myogenesis, neurogenesis, and lymphocyte development.
Ligand Receptor: Notch Receptors
Antigen References:

1. Ehebauer MT, et al. 2006. Biochem J. 392:13.
2. Shimizu K, et al. 2000. Mol Cell Biol. 20:6913.
3. Hellstrom M, et al. 2007. Nature 445:776.
4. Suchting S, et al. 2007. P. Natl. Acad. Sci. USA 104:3225.