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Safety Data Sheet (SDS) Purified Anti-mouse Jagged 2 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse Jagged 2 Antibody
1255005 50 µg $100.00       
Clone: HMJ2-1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: CHO cells expressing murine Jagged-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Moriyama Y, et al. 2008. Int. Immunol. 20:763 (FC)

FJ2/CHO transfectant cell line stained

FJ2/CHO transfectant cell line stained with HMJ2-1 purified, followed by anti-hamster IgG PE



Description:

The Notch receptors and their ligands are highly conserved from invertebrates to mammals. The Jagged-2 is one of the several Notch ligands identified. The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus. This translocation triggers a series of signaling process. Jagged-2 is reported to be essential for the regulation of spermatogenesis.

Other Names: HJ2, Jagged2, Jagged-2 precursor, JAG2
Structure: A single pass transmembrane protein consisting of a large extracellular N-terminal which includes the DSL domain, followed by a tandem array of EGF-like repeats (ELRs) and cysteine-rich domain (CRD). After a single transmembrane domain (TM) is a short in
Distribution: Testicular tissues and spermatids
Function: The Notch receptors and their ligands are highly conserved signaling molecules throughout development. They regulate cell fate decision for various cell development, including myogenesis, neurogenesis, and lymphocyte development. There is growing evidence that signals transmitted through different Notch ligands can differentially affect the target cells. Notch1/Jagged-2 signaling system plays an important role for male germ cells to differentiate.
Ligand Receptor: Notch receptors
Cellular Targets: Skeletrophin binds the intracellular regions of Jagged-2, but not those of Delta-1,3,4, or Jagged-1.
Antigen References:

1. Ehebauer MT, et al. 2006. Biochem J 392:13
2. Shimizu K, et al. 2000. Mol Cell Biology 20:18
3. Hellstrom M, et al. 2007. Nature 445:776
4. Luo B, et al. 1997. Mol Cell Biol. 17:6057