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Safety Data Sheet (SDS) FITC Anti-mouse H-2 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse H-2 Antibody
1227535 25 µg $85.00       
1227540 100 µg $180.00       
Clone: M1/42
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: C57BL/10 spleen cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

Stallcup KC, et al. 1981 J. Immunol. 127:923

C57BL/6 splenocytes stained with M1/42

C57BL/6 splenocytes stained with M1/42 FITC



Description:

The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KC et al, 1981). MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Other Names: Mouse major histocompatibility complex (MHC) H-2
Structure: 45kd heavy chain non-covalently associated with 12 kd beta-2 microglobulin
Distribution: All nucleated cells
Function: Present antigenic peptide to CD8+ cytotoxic T cells
Ligand Receptor: MHC/peptide
Antigen References:

1. Stallcup KC, et al. 1981. J. Immunol. 127:923