Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) Purified Anti-mouse CD194 CCR4 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD194 (CCR4) Antibody
1256010 100 µg $190.00       
Clone: 2G12
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Saito K, et al. 2008. J. Immunol. 181:6889. PubMed
2. Ueha S, et al. 2007. J. Leukoc. Biol. 82:1230. PubMed
3. Sharma R, et al. 2009 J. Immunol. 183:1065 (FC) PubMed
4. Dogan R, et al. 2011. J. Leukoc. Biol. 89:93. PubMed
5. Liu H, et al. 2011. Sci Transl Med. 3:82. PubMed.
6. Shankar SP, et al. 2012. J Immunol. 188:6347. PubMed.



Description:

Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85% identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naïve T cells, bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes and express cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity to migrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play an important role in the ligand-mediated recruitment of T cells into the skin in mice and humans, specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site of injury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucial for the induction and maintenance of self-tolerance and are present in peripheral tissues such as skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor and its ligand CCL22 (5).

Other Names: CC CKR-4, CHEMR1, Cmkbr4, LESTR, MGC151418, Sdf1r
Structure: GPCR, chemokine receptor, seven transmembrane receptor
Distribution: Memory T cells, macrophages, platelets, basophils, Th2 cells, and Tregs
Function: CCR4 and its ligands (CCL17 and CCL22) are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
Ligand Receptor: CCl17 and CCL22
Interaction: Interaction between CCR4 and its ligand TARC/CCL17 on activated endothelial cells mediates T cell extravasation by stimulating integrin-dependent adhesion
Antigen References:

1. Biederman T, et al. 2002. Eur. J. Immun. 32:3171.
2. Mirshahpanah P, et al. 2008. Exp. Dermatol. 17:30.
3. Kusumoto M, et al. 2007. J. Interferon Cytokine Res 27:901.
4. Clark RA and Kupper TS. 2006. Blood 109:194.
5. Lee I, et al. 2005. J. Exp. Med. 201:1037.