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Safety Data Sheet (SDS) FITC Anti-mouse CD94 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse CD94 Antibody
1127530 500 µg $245.00       
Clone: 18d3
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: CHO cells expressing the B6 alleles of CD94 and NKG2A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Tang X, et al. 2006. J. Immunol. 177:7645. PubMed
This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD49b (pan-NK cells) APC and CD94 (clone 18d3) FITC (top) or rat IgG2a, κ FITC isotype control (bottom).





Description:

CD94 is a 43/39 kD C-type lectin, also known as Kp43. It is present on all NK cells, NKT cells, and a subset of CD8-positive T lymphocytes in most mouse strains. CD94 is a type-II transmembrane protein with an extracellular lectin-like domain and a short cytoplasmic tail. CD94 is expressed as a disulphide-linked heterodimer with a NKG2 subunit believed to mediate signal transduction. When associated with NKG2A, the complex triggers inhibition; when associated with NKG2C, the complex triggers stimulation. The receptor complex of CD94 and NKG2 receptors bind to the ligand, Qa-1, and are thought to play a role in maintaining self-tolerance in developing NK cells.

Other Names: Kp43
Structure: C-type lectin; 43/39 kD
Distribution: NK cells, NK-T cells, CD8+ T subset
Function: Inhibits NK cell function
Ligand Receptor: CD94/NKG2
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165.
3. Moretta A, et al. 1997. Immunol. Rev. 155:105.
4. Phillips JH, et al. 1996. Immunity 5:163.