Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) PE Anti-mouse CD55 DAF Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD55 (DAF) Antibody
1259015 25 µg $100.00       
1259020 100 µg $245.00       
Clone: RIKO-3
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
C57BL/6 splenocytes stained with Riko-3

C57BL/6 splenocytes stained with Riko-3 PE



Description: CD55, also known as DAF (decay accelerating factor), is expressed on the surfaces of red blood cells, hematopoietic cells and many non-hemotopietic cells. There are two isoforms of mouse CD55, a GPI-anchored isofrom and a transmembrane isoform. Both are recognized by RIKO-3. The function of CD55 is to protect damage by complement deposition.
Other Names: Decay accelerating factor (DAF)
Structure: 60-70 kd single chain glycoprotein. While human CD55 contains only one CD55 gene, two distinct CD55 genes have been identified in the mouse, referred to as GPI-DAF and transmembrane(TM)-DAF. GPI-DAF is expressed only in testis.
Distribution: Red blood cells and most of the splenocytes, including B cells and T cells, and also many non-hematopoetic cells.
Function: Protect from complement attack by inhibition the deposition of C3 on antibody-sensitized cells.
Antigen References: 1. Miwa T et al. 2001. Immunology 104:207
2. Lin FY et al. 2001 Immunology 104:215