The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
C57BL/6 splenocytes stained with RA3-6B2 (B220) PE and Riko-3 Alexa Fluor® 647
CD55, also known as DAF (decay accelerating factor), is expressed on the surfaces of red blood cells, hematopoietic cells and many non-hemotopietic cells. There are two isoforms of mouse CD55, a GPI-anchored isofrom and a transmembrane isoform. Both are recognized by RIKO-3. The function of CD55 is to protect damage by complement deposition.
Decay accelerating factor (DAF)
60-70 kd single chain glycoprotein. While human CD55 contains only one CD55 gene, two distinct CD55 genes have been identified in the mouse, referred to as GPI-DAF and transmembrane(TM)-DAF. GPI-DAF is expressed only in testis.
Red blood cells and most of the splenocytes, including B cells and T cells, and also many non-hematopoetic cells.
Protect from complement attack by inhibition the deposition of C3 on antibody-sensitized cells.
1. Miwa T et al. 2001. Immunology 104:207 2. Lin FY et al. 2001 Immunology 104:215
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