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Safety Data Sheet (SDS) PE Anti-mouse CD276 B7-H3 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD276 (B7-H3) Antibody
1222535 25 µg $80.00       
1222540 100 µg $220.00       
Clone: RTAA15
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References: 1. Xu J, et al. 2006. Cellular and Molecular Immunology. 3(3):235
2. Prasad D.V.R. 2004. J.Immunology.173:2500
Thioglycolate-elicited BALB/c mouse peritoneal macrophages

Thioglycolate-elicited BALB/c mouse peritoneal macrophages stimulated with LPS for 24 hours and then stained with RTAA15 PE



Description: B7-H3 is a member of the B7 gene family. It has 88% amino acid sequence identity with human B7-H3. It is mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells. Its expression on dendritic cells appears to be up-regulated by LPS. B7-H3 protein inhibits T cell activation and cytokine production. It was reported that monoclonal antibody against B7-H3 enhanced T cell proliferation in vitro and led to exacerbated EAE in vivo. Therefore, B7-H3 may be a negative regulator on T cell activation and function.
Other Names: B7H3, B7RP-2
Structure: About 49.7 kd transmembrane protein characterized by extracellular IgV and IgC domains.
Distribution: Mouse B7-H3 is expressed on professional APCs, including B cells, dendritic cells, and macrophages. It is reported to express on a minor fraction of CD4+ and CD8+ cells.
Ligand Receptor: The receptor has not been identified, but it seems to be expressed on activated T cells.
Bioactivity/Activities: Mouse B7-H3 is a negative regulator of T cell activation and IL-2 production.
Antigen References: 1. Sun M et al. 2002. J. Immunology. 168:6294
2. Xu J et al. 2006. Cellular and Molecular Immunology. 3(3):235