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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse CD3 Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse CD3 Antibody
1101085 25 µg $95.00       
1101090 100 µg $270.00       
Clone: 17A2
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD)
2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete)
3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD)
4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
5. Zan H, et al. 2005. EMBO J. 24:3757.
6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
8. Molofsky AB, et al. 2013. J Exp Med. 210:535. PubMed
9. Al-Barwani F, et al. 2014. PLoS One. 9:104523. PubMed

C57BL/6 splenocytes stained with 17A2

C57BL/6 splenocytes stained with 17A2 PerCP/Cy5.5



Description:

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Other Names: T cell antigen receptor complex, T3
Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand Receptor: Peptide antigen/MHC-complex
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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