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Safety Data Sheet (SDS) TruStain FcX Anti-mouse CD16/32 Antibody     Product Data Sheet (PDF)    
TruStain FcX™ (anti-mouse CD16/32) Antibody
1106595 50 µg $50.00       
1106600 500 µg $190.00       
Clone: 93
Isotype: Rat IgG2a, λ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain fcX™ at 1.0 µg per 106 cells in 100 µl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.

Application References:

1. Oliver AM, et al. 1999. Hybridoma 18:113.
2. Brummel R and Lenert P. 2005. J. Immunol. 174:2429. PubMed
3. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349.
4. Clements JL, et al. 2006. J. Immunol. 177:905. PubMed
5. Flores M, et al. 2008. FASEB J. 22:3661. PubMed
6. Ge XN, et al. 2010. J. Immunol. 185:1205. PubMed
7. Maseda D, et al. 2012. J. Immunol. 188:1036. PubMed
8. Lewis ND, et al. 2013. J. Immunol. 190:3533. PubMed
9. Bonne-Annee S, et al. 2013. Infect Immun. 81:3346. PubMed
10. Parlane NA, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
11. Jarajapu YP, et al. 2014. PLoS One. 9:93965. PubMed
12. Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
13. Resa-Infante P, et al. 2014. J Virol. 88:8166. PubMed
14. Gillis TP, et al. 2014. Infect Immun. 82:3900. PubMed
15. Minkah N, et al. 2015. J Virol. 89:3366. PubMed



Description:

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain fcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.

Other Names: Fcγ R III/II, Ly-17
Structure: Ig superfamily, 40-60 kD
Distribution: B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
Function: Low affinity receptors for IgG
Ligand Receptor: IgG