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Safety Data Sheet (SDS) FITC Anti-rat RT1B Antibody     Product Data Sheet (PDF)    
FITC Anti-rat RT1B Antibody
1626525 100 µg $125.00       
Clone: OX-6
Isotype: Mouse IgG1, κ
Reactivity: Rat, Cross-Reactivity: Cross-reacts with mouse I-A[k] and I-A[s] alloantigens and with a major subset of splenocytes from NOD (I-A [g7]) mice.
Immunogen: Ia-like Glycoproteins from Wistar Thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% BSA (Origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Fox CC, et al. 1994. Cell Immunol. 158:253.
2. Chen-Woan M, et al. 1996. J. Leukocyte Biol. 59:196.
3. Damoiseaux JG, et al. 1998. J. Leukocyte Biol. 64:803.
4. De Riva A, et al. 2013. J. Immunol. 190:5961. PubMed

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-6 FITC and CD3 APC



Description:

RT1B is expressed on peripheral B lymphocytes, epidermal Langerhans cells, dendritic cells, some tissue macrophages, subsets of thymocytes, peritoneal mast cells, and small intestinal villus epithelium. The OX-6 antibody reacts with non-polymorphic determinants of the rat MHC class II antigen, I-A equivalent. The OX-6 monoclonal antibody cross-reacts with mouse I-A[k] and I-A[s] alloantigens and with a major subset of splenocytes from NOD (I-A [g7]) mice.

Other Names: MHC-class II, I-A equivalent
Structure: MHC-class II
Distribution: B lymphocytes, epidermal Langerhans cells, dendritic cells, some tissue macrophage, and subsets of thymocytes.
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD4