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Safety Data Sheet (SDS) Pacific Blue Anti-T-bet Antibody     Product Data Sheet (PDF)    
Pacific Blue™ Anti-T-bet Antibody
3824035 25 µg $150.00       
3824040 100 µg $320.00       
Clone: 4B10
Isotype: Mouse IgG1, κ
Reactivity: Human, Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

ICFC - Quality tested
IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set and the Nuclear Factor Fixation and Permeabilization Buffer Set .

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Application References:

1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.

Human peripheral blood lymphocytes were

Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) Pacific Blue™ (top) or mouse IgG1, κ Pacific Blue™ isotype control (bottom).





Description:

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Other Names: T-box expressed in T cells, T box 21, TBLYM
Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Antigen References:

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.