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Safety Data Sheet (SDS) FITC Anti-CD247 TCRzeta CD3zeta Antibody     Product Data Sheet (PDF)    
FITC Anti-CD247 (TCRζ, CD3ζ) Antibody
3820515 25 µg $100.00       
3820520 100 µg $260.00       
Clone: 6B10.2
Isotype: Mouse IgG1 κ
Reactivity: Human
Immunogen: Human TCR zeta chain aa38-54
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1.Zhang Z, et al. 2007. Blood 109:4328. (Block)
2.Gorman CL, et al. 2008. J. Immunol. 180:1060.
3.Jenson WA, et al. 1997. Eur J. Immunol. 27:707.
4. Mao H, et al. 2010. J. Virol. 84:4148. PubMed
5. Hwang I, et al. 2012. Int Immunol. 24:793. PubMed.

Human peripheral blood lymphocytes surface

Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) PE then intracellular stained with 6B10.2 FITC



Description:

The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR heterodimer. The disulfide-linked TCR zeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.

Other Names: CD247, CD3zeta
Structure: 163 amino acid long protein with a short extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
Distribution: T cells and NKT cells. Reported also positive on CD16+ human NK cells.
Function: Mediating signal transduction after TCR receptor engagement.
Interaction: ZAP70, TCR alpha/beta
Antigen References:

1.Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709