The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
1. Siegel RM, et al. 2001. Nat. Immunol. 2:577. 2. Von Bulow GU, et al. 1997. Science 278:138. 3. Yan M, et al. 2001. Nat. Immunol. 2:638. 4. Diaz-de-Durana Y, et al. 2006. Blood 107:594. 5. Moir S, et al. 2012. Blood. 120:4850. PubMed.
C57BL/6 splenocytes stained with 8F10 PE and RA3-6B2 APC
Transmembrane Activator and CAML Interactor (TACI) is a tumor necrosis factor receptor superfamily (TNFSF) member. It is a type III transmembrane protein sharing a high degree of homology with B cell maturation antigen (BCMA) among TNFSF members. It binds to the ligands BAFF (BLyS) and APRIL. CD267 appears to be particularly important for the regulation of B cell survival and maturation in the spleen. It is a major mediator of BAFF-dependent costimulatory responses in peripheral B cells. Signaling through TACI may serve to down-regulate B cell activity as well. The actual role for TACI in the mouse has still not been fully determined.
TACI/CD267 is a type III transmembrane protein belonging to TNF receptor superfamily (TNFSF). Among TNFSF members, TACI shares a high degree of homology with B cell maturation antigen (BCMA). It binds to the ligands BAFF (BLyS) and APRIL.
CD267 is expressed predominantly by splenic transitional type 2 and marginal zone B cells, as well as activated B cells. But it is notably absent from germinal center B cells.
CD267 appears to be particularly important for the regulation of B cell survival and maturation in the spleen. It is a major mediator of BAFF-dependent costimulatory responses in peripheral B cells. Signaling through TACI may serve to down-regulate B cell activity.
BAFF (Blys/TALL-1/THANK) and APRIL
1. Ng LG, et al. 2004. J. Immunology. 173:807.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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