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Safety Data Sheet (SDS) PE Anti-mouse CD226 DNAM-1 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD226 (DNAM-1) Antibody
1268015 25 µg $95.00       
Clone: TX42.1
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse DNAM-1 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Dardalhon V, et al. 2005. J. Immunol. 175:1558
2. Shirakawa J, et al. 2006. International Immunology 18(6):951-957

C57BL/6 splenocytes stained with CD8

C57BL/6 splenocytes stained with CD8 (53-6.7) APC and TX42 PE



Description:

Mouse CD226 (DNAM-1) is a 65 kd glycoprotein of a member of Ig super-family adhesion molecule containing 2 immunoglobulin-like domains. It is associated with leukocyte function associated antigen-1 (LFA-1) involving in TCR-mediated signal transduction for T cell proliferation and differentiation. CD226 is also involved in NK cell and T cell mediated cytotoxicity against certain tumors. CD226 (DNAM-1) is constitutively expressed on naïve CD8+ and CD4+ T cells, macrophages, and NK cells. It was reported that CD226 expression is up-regulated on Th1 cells, but down-regulated on Th2 and Th0 cells. CD155 and CD112 are the ligands for CD226.

Other Names: DNAM-1, PTA1 (Platelet and T cell activation antigen 1), TLISA1, LFA-1 associated molecule PTA-1
Structure: 65 kd glycoprotein of a member of Ig super-family containing 2 immunoglobulin-like domains.
Distribution: Expressed on majority of CD8+ and CD4+ T cells, NK cells, monocytes/macrophages and a subset of B cells. Reported to up-regulated on Th1 cells, but down-regulated in Th2 cells.
Function: Mediate cellular adhesion, involved in LFA-1 co-stimulatory signal for T cell differentiation and proliferation.
Ligand Receptor: CD112 and CD155
Antigen References:

1 Tahara Hanaoka S, et al. 2006. Blood. 107:1491
2 Shibuya K et al. 2003. J. Exp. Med. 198:1829.