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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD366 Tim-3 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD366 (Tim-3) Antibody
1270025 25 µg $100.00       
1270030 100 µg $220.00       
Clone: B8.2C12
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: mTim-3 protein/Freund adjuvant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Clone B8.2C12 only binds to the BALB/c allele of Tim-3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

Mouse CD366 (Tim-3) transfected cells

Mouse CD366 (Tim-3) transfected cells stained with anti-mouse CD366 (Tim-3, clone B8.2C12) Alexa Fluor® 647



Description:

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Other Names: T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain. It can exist as a soluble form lacking mucin and transmembrane domains.
Distribution: Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.
Function: Play a role in regulating macrophage activation, T cell apoptosis and immune tolerance.
Ligand Receptor: Galectin -9
Antigen References:

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454
3. Mooney L, et al. 2002. Nature. 415:536
4. Rodriguez-Manzanet R, et al. 2009. Immunol. Rev. 229(1):259


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