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Safety Data Sheet (SDS) PE Anti-mouse IL-9 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse IL-9 Antibody
3170515 25 µg $150.00       
3170520 100 µg $280.00       
Clone: RM9A4
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Recombinant full length mouse IL-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The IL-9 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

See the protocol for Th9 polarization of mouse splenocytes.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Angkasekwinai P, et. al. 2010. Nat. Immunol. 11:250. (ICFC) PubMed
2. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ICFC) PubMed
3. Takami M, et al. 2012. J Immunol. 188:4369. PubMed
4. Reynolds LA, et al. 2012. J. Immunol. 189:1113. PubMed
5. Kuwahara M, et al. 2014. Nat Commun. 5:3555. PubMed





FACS sorted naive CD4 T

FACS sorted naive CD4 T cells (CD44lo, CD25-) were cultured on plates coated with 1 µg/ml anti-CD3 and 10 µg/ml anti-CD28 in the presence of 10 ng/ml IL-4 and 5 ng/ml TGFβ for 3 days. Intracellular staining was performed following a 4h restimulation with PdBU/ionomycin/brefeldin. Data kindly provided by: Dr. Brigitta Stockinger, PhD



Description:

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones.

Other Names: Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Structure: Cytokine; 30-40 kD (Mammalian)
Regulation: Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Cellular Sources: IL-2 activated TH2 lymphocytes
Cellular Targets: T lymphocytes, erythroid precursors
Receptors: IL-9R
Bioactivity/Activities: Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Antigen References:

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V, et al. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y, et al. 1992. Leuk. Lymphoma 8:441.