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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD218a IL-18Ralpha Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD218a (IL-18Rα) Antibody
1264515 25 tests $150.00       
Clone: BG/IL18RA
Isotype: Rat IgG1
Reactivity: Mouse
Immunogen: Mouse NS0-derived rmIL-18Rα extracellular domain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Yu Q, et al. 2008. J. Immunol. 181:3346
2. Pham D, et al. 2012. J. Immunol. 189:832. PubMed
3. Tyznik AJ, et al. 2014. J. Immunol. 192:3676. PubMed

C57BL/6 splenocytes stained with CD3

C57BL/6 splenocytes stained with CD3 (145-2C11) PE and BG/IL18RA Alexa Fluor® 647



Description:

The IL-18 receptor (IL-18R) complex is composed of two known chains, IL-18 receptor α (IL-18Rα) and IL-18 receptor β (IL-18Rβ). IL-18Rα (IL-1R5) is an IL-1 receptor super family member. It was originally described as IL-1 receptor-related protein (IL-1Rrp) because of its homology with the IL-1/Toll receptor family. IL-18Rα is the extracellular binding domain of the IL-18 receptor complex. It is expressed on the majority of peripheral CD4+ T cells, Th1 cells, and reciprocally repressed during Th2 development. The expression of IL-18Rα is induced by an IL-12/Stat4 pathway and inhibited by IL-4/Stat6 pathway. Binding of IL-18Rα together with IL-18β to proinflammatory cytokine IL-18 plays an important role in innate and autoimmune responses.

Other Names: IL-1 Rrp, CDw218a, IL-18 receptor a
Structure: An IL-1 receptor super family member
Distribution: Expressed on T cells, NK cell subset, neutrophils, IL-12 stimulated tonsillar B cells, and intestinal epithelial cells (IEC).
Function: Bind to pro inflammatory cytokine IL-18 and play an important role in innate and autoimmune responses.
Ligand Receptor: IL-18
Antigen References:

1. Parnet P, et al. 1996. J. Biol. Chem. 271 :3967
2. Thomassen E, et al. 1998. J. Interferon Cytokine Res. 18:1077
3. Debets R, et al. 2000. J. Immunol. 165:4950
4. Smeltz RB, et al. 2001. J. Exp. Med. 194:143


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