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Safety Data Sheet (SDS) PE Anti-mouse B7-H4 B7S1 B7X Antibody     Product Data Sheet (PDF)    
PE Anti-mouse B7-H4 (B7S1, B7X) Antibody
1240535 50 µg $105.00       
Clone: clone 9
Isotype: Rat IgG1
Reactivity: Mouse
Immunogen: B7-S1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

Mouse B7-H4 transfected 293E cells

Mouse B7-H4 transfected 293E cells stained with clone 9 PE



Description:

B7-H4 is a newly discovered B7 family member that negatively regulates T cell immunity by inhibiting of T cell proliferation, cytokine production, and cell cycle progression. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production, and generation of alloreactive cytotoxic T-cells (CTLs). In vivo, blockade of endogenous B7-H4 by specific monoclonal antibody promotes T cell responses. A new function of B7-H4 was reported as an important negative regulator of innate immunity through growth inhibition of neutrophils. It was reported that B7-H4 does not constitutively expressed on peripheral tissues but could be induced to express on T cells, B cells, macrophages, and dendritic cells. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance. Although B- and T- lymphocyte attenuator (BTLA) was proposed to be the receptor for B7-H4, further studies are needed to identify the inhibitory receptor of B7-H4.

Other Names: B7H4, B7S1, B7-S1, B7x
Structure: Belong to the B7 family of costimulatory proteins and contain two extracellular Ig-like domains.
Distribution: Induced to express on T cells, B cells, macrophages, and dendritic cells. It is also expressed on some tumor cancer cells.
Function: Inhibit T cell activation, cell cycle progression, and cytokine production.
Ligand Receptor: A putative receptor on T cells