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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD2442 2B4 B6 Alloantigen Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD244.2 (2B4 B6 Alloantigen) Antibody
1267545 25 µg $105.00       
1267550 100 µg $215.00       
Clone: m2B4 (B6)458.1
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Immunogen: m2B4-Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The 2B4 antibody reacts with CD244.2, the 2B4 allloantigen which is expressed on C57BL/6 mice, but not on BALB/c mice.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Application References:

1. Lee KM, et al. 2006. Blood. 107:3181.
2. Schatzle JD, et al. 1999. P. Natl Acad Sci USA. 96:3870.
3. Stepp SE, et al. 1999. Eur. J. Immunol. 29:2392.
4. de la Luz Sierra M, et al. 2010. Blood. 115:3970. PubMed

C57BL/6 splenocytes stained with m2B4(B6)458.1

C57BL/6 splenocytes stained with m2B4(B6)458.1 Alexa Fluor® 647 (upper panel) or mouse IgG1 isotype control Alexa Fluor® 647 (lower panel) and anti-mouse CD49b (DX5) PE





Description:

Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non- MHC-restricted cytotoxicity. It is expressed on all natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes, including dendritic epidermal T cells. There are at least two isoforms of CD244.2 proteins that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner, while the short form functions in an activating manner. The ligand of CD244 is CD48, which is expressed on all hematopoietic cells. It was reported that CD244 interaction with CD48 is essential for IL-2-driven expansion and activation of murine NK cells.

Other Names: 2B4 B6 Alloantigen
Structure: A member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).
Distribution: All natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes.
Function: Mediate non-MHC-restricted cytotoxicity.
Ligand Receptor: CD48
Antigen References:

1. Brown MH, et al. 1998. J. Exp. Med. 188:2083.
2. Davis SJ, et al. 1996. Immunol Today. 17:177.
3. Kumaresan PR, et al. 2000. Immunogenetics. 51:306.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.


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