The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested IHC - Reported in the literature
Additional reported applications (for relevant formats of this clone) include:depletion2, immunohistochemistry of frozen sections (OCT embedded2).
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Obata K, et al. 2007. Blood 110:913 (FC) 2. Sokol CL, et al. 2008. Nat. Immunol. 9:310(FC, Deplete, IHC) 3. Chen J, et al. 2009. J. Biol. Chem.. 284:5763 (FC)
Mouse mast cell line MC/9 stained with purified MAR-1 conjugated to PE
FcεRIα is a transmembrane protein belonging to the Ig superfamily. FcεRIα forms a tetrameric complex with one β and two γ-subunits. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions. It is abundantly expressed on mast and basophils and up-regulated by the presence of IgE. Following stimulation via FcεRIα, mast cells and basophils release bioactive chemical mediators such as histamine, resulting in the initiation of allergic reactions. Cross linking of the high-affinity receptor for IgE on tissue mast cells triggers immediate hypersensitivity with local symptoms. The MAR-1 monoclonal antibody reacts with the FcεRIα subunit.
FceRIa, FceRI-α, high affinity IgE receptor
A transmembrane protein of Ig super family member
Expressed on mast and basophils.
Play an important role in triggering IgE-mediated allergic reactions.
1. Arinobu Y, et al. 2005. P. Natl. Acad. Sci. USA 102:18105. 2. Yamaguchi M, et al. 2001. Int. Immunol. 13:843.
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