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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse FcepsilonRIalpha Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse FcεRIα Antibody
1271545 25 µg $120.00       
1271550 100 µg $260.00       
Clone: MAR-1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for relevant formats of this clone) include: depletion2, immunohistochemistry of frozen sections (OCT embedded2).

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Application References:

1. Obata K, et al. 2007. Blood 110:913 (FC)
2. Sokol CL, et al. 2008. Nat. Immunol. 9:310 (FC, Deplete, IHC)
3. Chen J, et al. 2009. J. Biol. Chem.. 284:5763 (FC)

Mouse mast cell line MC/9

Mouse mast cell line MC/9 stained with MAR-1 Alexa Fluor® 647



Description:

FcεRIα is a transmembrane protein belonging to the Ig superfamily. FcεRIα forms a tetrameric complex with one β and two γ-subunits. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions. It is abundantly expressed on mast and basophils and up-regulated by the presence of IgE. Following stimulation via FcεRIα, mast cells and basophils release bioactive chemical mediators such as histamine, resulting in the initiation of allergic reactions. Cross linking of the high-affinity receptor for IgE on tissue mast cells triggers immediate hypersensitivity with local symptoms. The MAR-1 monoclonal antibody reacts with the FcεRIα subunit.

Other Names: FceRIa, FceRI-α, high affinity IgE receptor
Structure: A transmembrane protein of Ig super family member
Distribution: Expressed on mast and basophils.
Function: Play an important role in triggering IgE-mediated allergic reactions.
Ligand Receptor: IgE
Antigen References:

1. Arinobu Y, et al. 2005. P. Natl. Acad. Sci. USA 102:18105.
2. Yamaguchi M, et al. 2001. Int. Immunol. 13:843.


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