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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-rat CD44H Pgp-1 H-CAM Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-rat CD44H (Pgp-1, H-CAM) Antibody
1619540 100 µg $220.00       
Clone: OX-49
Isotype: Mouse IgG2a, κ
Reactivity: Rat
Immunogen: Rat T blasts from mixed lymphocyte reactions
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Paterson DJ, et al. 1987. Mol Immunol. 24(12):1281

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-49 Alexa Fluor® 647



Description:

CD44 is an 80-95 kD cell surface glycoprotein. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. It is up-regulated when T cells and B cells are activated. It was reported that CD44 is a valuable marker for memory T cells. CD44 is an adhesion molecule involved in leukocyte adhesion and homing to lymphoid organs. The OX-49 antibody reacts with CD44H (known as CD44s) expressed on most leukocytes, except for a subset of B lymphocytes. The epitope recognized by OX-49 antibody has been mapped to a region on both the standard, CD44s, and the splice variant, CD44v, isoforms of CD44. However it was reported that OX-49 antibody cannot detect the CD44V isoform, possibly due to conformational changes in the epitope.

Other Names: Pgp-1, H-CAM, CD44s
Structure: A 80-95 kD cell surface glycoprotein
Distribution: Expressed on most leukocytes. It is up-regulated when T cells and B cells are activated.
Function: Involve in leukocyte adhesion and homing to lymphoid organs.
Ligand Receptor: Hyaluronate
Antigen References:

1. Arch R, et al. 1992. Science 257 (5070):682
2. Mitnacht R, et al. 1995. Eur. J. Immunol. 25(2):328
3. Zheng Z, et al. 1995. J Cell Bio. 130(2):485
4. Westermann J, et al. 1994. Eur. J. Immunol. 24(10):2312


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