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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD268 BAFF-R Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD268 (BAFF-R) Antibody
1270525 25 µg $130.00       
1270530 100 µg $265.00       
Clone: 7H22-E16
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Mouse BAFF receptor transfected rat RBL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Ng LG, et al. 2006. Eur. J. Immunol. 36:1837.
2. Ng LG, et al. 2004. J Immunol. 173:807.
3. Batten M, et al. 2000. J. Exp. Med. 192:1453.
4. Fu L, et al. 2009. Blood 113:4627.
5. Zhou X, et al. 2013. Mol Cell Biol. 33:2091. PubMed.

C57BL/6 splenocytes double stained with

C57BL/6 splenocytes double stained with 7H22-E16 Alexa Fluor® 647 (upper panel) or rat IgG1 isotype control Alexa Fluor® 647 (lower panel) and B220 (RA3-6B2) PE


BAFF (B cell-activating factor belonging to the TNF family) receptor (BAFF-R) is one of the three receptors that bind to BAFF. It is a type III transmembrane TNF receptor family member. BAFF-R is expressed at high levels in resting B cells and at lower levels in activated B cells, a small subset of activated/memory CD4+ T cells. It is a major mediator of BAFF dependent co-stimulatory response in peripheral B and T cells. It was reported that the mice expressing defective BAFF-R have disrupted B cell maturation. BAFF receptor appears to be particularly important for the regulation of normal B cell survival and maturation, as well as in the pathophysiology of aggressive B-cell malignancies and autoimmune diseases.

Other Names: BR3, BAFFR, BAFF receptor, TNFRSF13C, B cell-activating factor receptor, BLys receptor 3
Structure: A type III transmembrane TNF receptor family member
Distribution: Expressed on most B cells, a small subset of CD4+ T cells.
Function: Play an importance role in B cell development, maturation, and survival.
Ligand Receptor: BAFF (BLyS)
Antigen References:

1. Thompson JS, et al. 2001. Science 293:2108.
2. Schiemann B, et al. 2001. Science 293:2111.
3. Batten M, et al. 2000. J. Exp. Med. 192:1453.
4. Fu L, et al. 2009. Blood 113:4627.

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.