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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD268 BAFF-R Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD268 (BAFF-R) Antibody
1270525 25 µg $130.00       
1270530 100 µg $265.00       
Clone: 7H22-E16
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Mouse BAFF receptor transfected rat RBL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Ng LG, et al. 2006. Eur. J. Immunol. 36:1837.
2. Ng LG, et al. 2004. J Immunol. 173:807.
3. Batten M, et al. 2000. J. Exp. Med. 192:1453.
4. Fu L, et al. 2009. Blood 113:4627.
5. Zhou X, et al. 2013. Mol Cell Biol. 33:2091. PubMed.

C57BL/6 splenocytes double stained with

C57BL/6 splenocytes double stained with 7H22-E16 Alexa Fluor® 647 (upper panel) or rat IgG1 isotype control Alexa Fluor® 647 (lower panel) and B220 (RA3-6B2) PE





Description:

BAFF (B cell-activating factor belonging to the TNF family) receptor (BAFF-R) is one of the three receptors that bind to BAFF. It is a type III transmembrane TNF receptor family member. BAFF-R is expressed at high levels in resting B cells and at lower levels in activated B cells, a small subset of activated/memory CD4+ T cells. It is a major mediator of BAFF dependent co-stimulatory response in peripheral B and T cells. It was reported that the mice expressing defective BAFF-R have disrupted B cell maturation. BAFF receptor appears to be particularly important for the regulation of normal B cell survival and maturation, as well as in the pathophysiology of aggressive B-cell malignancies and autoimmune diseases.

Other Names: BR3, BAFFR, BAFF receptor, TNFRSF13C, B cell-activating factor receptor, BLys receptor 3
Structure: A type III transmembrane TNF receptor family member
Distribution: Expressed on most B cells, a small subset of CD4+ T cells.
Function: Play an importance role in B cell development, maturation, and survival.
Ligand Receptor: BAFF (BLyS)
Antigen References:

1. Thompson JS, et al. 2001. Science 293:2108.
2. Schiemann B, et al. 2001. Science 293:2111.
3. Batten M, et al. 2000. J. Exp. Med. 192:1453.
4. Fu L, et al. 2009. Blood 113:4627.


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