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Safety Data Sheet (SDS) Pacific Blue Anti-human/mouse CD49f Antibody     Product Data Sheet (PDF)    
Pacific Blue™ Anti-human/mouse CD49f Antibody
2168095 25 tests $115.00       
2168100 100 tests $245.00       
Clone: GoH3
Isotype: Rat IgG2a, κ
Reactivity: Human, Mouse, Cross-Reactivity: Baboon, Chimpanzee, Capuchin Monkey, Cynomolgus, Rhesus, Horse (Equine), Cattle (Bovine, Cow), Sheep (Ovine), Swine (Pig, Porcine), Dog (Canine), Cat (Feline), Rabbit (Lapine)
Immunogen: Mouse mammary tumor cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin α61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin α6 function in vivo.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Application References:

1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
15. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed

Human peripheral blood lymphocytes stained

Human peripheral blood lymphocytes stained with GoH3 Pacific Blue™



Description:

CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.

Other Names: VLA-6 α chain, α6 integrin, integrin α6, ITGA6
Structure: Integrin family, associates with β1 or β4, 120 kD
Distribution: Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells
Function: Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
Ligand Receptor: With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
Antigen References:

1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.