The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
See the protocol for Th9 polarization of mouse splenocytes.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Angkasekwinai P, et. al. 2010. Nat. Immunol. 11:250. (ICFC) PubMed 2. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ICFC) PubMed 3. Takami M, et al. 2012. J Immunol. 188:4369. PubMed. 4. Reynolds LA, et al. 2012. J. Immunol. 189:1113. PubMed.
CD4+ T cells from C57BL/6 mouse were cultured with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-beta for three days. Intracellular staining was performed following 5 hour restimulation with PdBU, Ionomycin, and brefeldin
IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones.
Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
IL-2 activated TH2 lymphocytes
T lymphocytes, erythroid precursors
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Quesniaux V, et al. 1992. Research Immunology 143:385. 3. Renauld J, et al. 1993. Adv. Immunol. 54:79. 4. Yang Y, et al. 1992. Leuk. Lymphoma 8:441.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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