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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse IL-9 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse IL-9 Antibody
3170535 25 µg $155.00       
Clone: RM9A4
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Recombinant full length mouse IL-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

See the protocol for Th9 polarization of mouse splenocytes.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Angkasekwinai P, et. al. 2010. Nat. Immunol. 11:250. (ICFC) PubMed
2. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ICFC) PubMed
3. Takami M, et al. 2012. J Immunol. 188:4369. PubMed.
4. Reynolds LA, et al. 2012. J. Immunol. 189:1113. PubMed
5. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed

CD4+ T cells from C57BL/6

CD4+ T cells from C57BL/6 mouse were cultured with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-beta for three days. Intracellular staining was performed following 5 hour restimulation with PdBU and ionomycin (in the presence of brefeldin A)





Description:

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones.

Other Names: Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Structure: Cytokine; 30-40 kD (Mammalian)
Regulation: Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Cellular Sources: IL-2 activated TH2 lymphocytes
Cellular Targets: T lymphocytes, erythroid precursors
Receptors: IL-9R
Bioactivity/Activities: Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Antigen References:

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V, et al. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y, et al. 1992. Leuk. Lymphoma 8:441.


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