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Safety Data Sheet (SDS) PE Anti-mouse CD66a CEACAM1a Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD66a (CEACAM1a) Antibody
1272525 25 µg $95.00       
1272530 100 µg $245.00       
Clone: MAb-CC1
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Immunogen: Balb/c mouse purified intestinal brush border membrane
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Turner BC, et al. 2004. J. Virol. 78 (10):5486
2. Williams RK, et al. 1990. J. Virol. 64:3817
3. Dveksler GS, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:1716

Balb/c mouse splenocytes stained with

Balb/c mouse splenocytes stained with MAb-CC1 PE (lower panel) or mouse IgG1 PE isotype control (upper panel) and CD45R/B220 (RA3-6B2) APC





Description:

CD66a, known as CEACAM1a, carcinoembryonic antigen-related cell adhesion molecule 1a, is a glycoprotein of the immunoglobulin superfamily and the carcinoembryonic antigen family. Isoforms expressing either two or four alternatively spliced Ig-like domains in mice have been found in a number of epithelial, endothelial, or hematopoietic tissues. CEACAM1a functions as an intercellular adhesion molecule, an angiogenic factor, and a tumor cell growth inhibitor. It also serves as a signal regulatory protein influencing B cell receptor complex-mediated activation. The mouse and human CEACAM1a proteins are targets of viral or bacterial pathogens, respectively. It was reported that targeted disruption of the CEACAM1a gene resulting in a partial ablation of the protein in mice led to reduced susceptibility to virus infection. The antibody recognizes the N-terminal domain of murine CEACAM1a, it does not recognize murine CEACAM1b, an allele in SJL mice.

Other Names: CEACAM1a, Bgp
Structure: A glycoprotein of the immunoglobulin (Ig) superfamily and the carcinoembryonic antigen family
Distribution: CEACAM1a is abundantly expressed on B cells, granulocytes, some NK cells, monocytes, DCs, and NK cells. It is found on epithelial and endothelial cells.
Function: CEACAM1a is involved in cell adhesion, tumor growth, and viral infection. It regulates on BCR activation of B lymphocytes.
Antigen References:

1. Nakagaki K, et al. 2005. J. Virol. 79(10):6102
2. Greicius G et al. 2003. J. Leukoc. Biol. 74(1):126
3. Hemmila E et al. 2004. J. Virol. 78(18):10156