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Safety Data Sheet (SDS) Purified Anti-mouse CD146 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD146 Antibody
1273505 50 µg $90.00       
1273510 200 µg $260.00       
Clone: ME-9F1
Isotype: Rat IgG2a
Reactivity: Mouse
Immunogen: Endothelial cell line TME-3H3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Schrage A, et al. 2008. Histochem. Cell. Biol. 129:441.
2. Birbrair A, et al. 2013. Am J Physiol Cell Physiol. 305:1098. PubMed

C57BL/6 splenocytes stained with ME-9F1

C57BL/6 splenocytes stained with ME-9F1 PE and NK-1.1 (PK136) APC

C57BL/6 splenocytes stained with rat

C57BL/6 splenocytes stained with rat IgG2a (RTK2758) PE isotype control and NK-1.1 (PK136) APC



Description:

CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30% of neutrophils and 60% of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFNγ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.

Other Names: S-Endo 1 antigen, MUC18, MCAM, Mel-CAM, A32 antigen
Structure: CD146 is a transmembrane glycoprotein and belongs to the Ig superfamily of cell adhesion molecules.
Distribution: CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1+ cells, and neutrophils.
Function: CD146 has been reported to be functionally relevant for endothelial cell adhesion, angiogenesis, and NK cell function.
Ligand Receptor: Unidentified ligand.
Antigen References:

1. Despoix N, et al. 2008. Eur. J. Immunol. 38:2855.
2. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035.
3. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746.