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Safety Data Sheet (SDS) PE Anti-rat CD200 Antibody     Product Data Sheet (PDF)    
PE Anti-rat CD200 Antibody
1624035 100 µg $170.00       
Clone: OX-2
Isotype: Mouse IgG1, κ
Reactivity: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

1. Dick, A.D., et al. 2001. Invest Ophthalmol. Vis. Sci. 42:170.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-2 PE and RT1D (OX-17) FITC

LOU rat splenocytes double stained

LOU rat splenocytes double stained with mouse IgG1 (MOPC-21) PE isotype control and RT1D (OX-17) FITC



Description:

CD200, known as OX-2, is a type I membrane glycoprotein member of the Ig supergene family. CD200 is expressed on B cells, a subset of T cells, thymocytes, follicular dendritic cells, neurons, keratinocytes, vascular endothelium, and some smooth muscle. The interaction of CD200 with CD200 receptor provide a potent costimulatory T-cell signal in the presence of TCR signaling, stimulate macrophages, and inhibit mast cell degranulation. It was reported that increased expression of OX-2 on DC was associated with inhibition of cytokine production and renal allograft rejection. Incubation of lymphocytes with OX-2 Fc inhibits a primary mixed lymphocyte reaction in vitro, decreased IL-2 and IFN-γ production, increased IL-4 and IL-10 production. In vivo infusion of OX-2 Fc promotes both skin and renal graft survival and decreases the antibody response. The OX-2 antibody reacts with rat OX-2 antigen.

Other Names: OX-2, OX2
Structure: A 47kD (thymus) or 41-kD (brain) type I membrane glycoprotein member of the Ig supergene family. CD200 molecule contains two extracellular Ig-like domains, an Ig V-like, and an Ig C-like domain.
Distribution: CD200 is expressed on B cells, a subset of T cells, thymocytes, follicular dendritic cells, neurons, vascular endothelium, and some smooth muscle.
Function: The interaction of CD200 with CD200 receptor provide a potent costimulatory T-cell signal in the presence of TCR signaling, stimulate macrophages, and inhibit mast cell degranulation.
Ligand Receptor: CD200 receptor
Antigen References:

1. McMaster WR, et al. 1979. Eur. J. Immunol. 9:426
2. Barclay A. N, et al. 1981. Immunology. 44:727
3. Bukovsky A, et al. 1984. Immunol. 52:631
4. Borriello F, et al. 1997. J. Immunol. 158:4548
5. Gorczynski R. M, et al. 1999. J. Immunol. 163:1654
6. Gorczynski L, et al. 1999. J. Immunol. 162:774