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Safety Data Sheet (SDS) APC Anti-mouse TCR Gamma/delta Antibody     Product Data Sheet (PDF)    
APC Anti-mouse TCR γ/δ Antibody
1190575 25 µg $90.00       
1190580 100 µg $135.00       
Clone: GL3
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: C57BL/6J intraepithelial lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ T cells3-5.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
2. Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
3. Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
4. Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
5. Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
6. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
7. Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
8. Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
9. Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
10. Pai MH, et al. 2014. JPEN J Paramerer Enteral Nutr. PubMed

C57BL/6 mouse lymph node cells

C57BL/6 mouse lymph node cells stained with CD3 (145-2C11) PE and GL-3 APC

C57BL/6 mouse lymph node cells

C57BL/6 mouse lymph node cells stained with CD3 (145-2C11) PE and Armenian hamster IgG APC isotype control



Description:

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, which is involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8- although some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.

Other Names: T cell receptor γ/δ
Structure: Ig superfamily, associates with CD3 complex.
Distribution: T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
Function: Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
Ligand Receptor: Some bacterial or tumor antigens bound to MHC class I.
Antigen References:

1. Skarstein K, et al. 1995. Immunology 81:497.
2. Harrison LC, et al. 1996. J. Exp. Med. 184:2167.
3. Wildner G, et al. 1996. Eur. J. Immunol. 26:2140.
4. Brandes M, et al. 2005. Science 309:264.