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Safety Data Sheet (SDS) FITC Anti-human/mouse Granzyme B Antibody     Product Data Sheet (PDF)    
FITC Anti-human/mouse Granzyme B Antibody
3177015 25 tests $115.00       
Clone: GB11
Isotype: Mouse IgG1, κ
Reactivity: Human, Mouse, Cross-Reactivity: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Wever PC, et al. 1998. Immunology. 93:383
2. Arens R, et al. 2004. J. Exp. Med. 199:1595
3. Lima M, et al. 2003. Am. J. Pathol. 163:763
4. Wiede F, et al. 2014. J Autoimmun. 53:105. PubMed
5. Baker GF, et al. 2014. Cancer Res. 74:5079. PubMed
6. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
7. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed

Human peripheral blood lymphocytes surface

Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with GB11 FITC

Human peripheral blood lymphocytes surface

Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with mouse IgG1 FITC isotype control



Description:

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Other Names: Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
Structure: 32 kD serine protease
Distribution: Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells
Function: Induction of cell death and apoptosis
Ligand Receptor: Mannose-6-phosphate receptor
Antigen References:

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761