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Safety Data Sheet (SDS) Pacific Blue Anti-mouse CD23 Antibody     Product Data Sheet (PDF)    
Pacific Blue™ Anti-mouse CD23 Antibody
1108075 25 µg $100.00       
1108080 100 µg $220.00       
Clone: B3B4
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, and immunohistochemical staining of acetone-fixed frozen sections5.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Application References:

1. Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
2. Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
3. Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
4. Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
5. Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
6. Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
7. Joly MS, et al. 2014. J Immunol. 193:3947. PubMed

CD57BL/6 mouse splenocytes were stained

CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) Pacific Blue™ (orange histogram), or rat IgG2a, κ Pacific Blue™(blue histogram).



Description:

CD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.

Other Names: FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6
Structure: C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution: B cells, follicular dendritic cells
Function: Regulates B cell activation
Ligand Receptor: IgE, CD19/CD21/CD81
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.