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Safety Data Sheet (SDS) Pacific Blue Anti-mouse CD105 Antibody     Product Data Sheet (PDF)    
Pacific Blue™ Anti-mouse CD105 Antibody
1202055 25 µg $90.00       
1202060 100 µg $190.00       
Clone: MJ7/18
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: inflamed mouse skin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications include: immunoprecipitation, Western blotting, and immunofluorescence histochemistry or immunohistochemistry of acetone-fixed frozen sections2-4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Application References:

1. Ge AZ and Butcher EC. 1994. Gene 138:201.
2. Baluk P, et al. 2003. Am. J. Pathol. 163:1801. (IHC)
3. Takahashi T, et al. 2003. Mol. Cell Biol. 23:1817. (IHC)
4. Savinov AY, et al. 2003. J. Exp. Med. 197:643. (IHC)

bEND.3 cells stained with MJ7/18

bEND.3 cells stained with MJ7/18 Pacific Blue™



Description:

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and upregulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF-β1, TGF-β3 and modulates TGF-β signaling by interacting with TGF-β receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2, and BMP-7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging and prognosis, and has therapeutic potential for some solid tumors and other angiogenic diseases.

Other Names: Endoglin
Structure: Type I integral membrane protein, homodimer, TGF-β type III receptor family member
Distribution: Endothelial cells, activated monocytes, macrophages, stromal cells, some cytotrophoblast
Function: Suppresses TGF-β signaling, angiogenesis
Ligand Receptor: TGF-β1, TGF-β3
Antigen References:

1. Gougos A and M. Letarte 1988. J. Immunol. 141:1925.
2. Cheifetz S, et al. 1992. J. Bio. Chem. 267:19027.
3. Barbara NP, et al. 1999. J. Bio. Chem. 274:584.
4. Lastres P, et al. 1992. Eur. J. Immunol. 22:393.
5. Duff S, et al. 2003. FASEB J. 17:984.
6. Warrington K, et al. 2005. Anticancer Res. 25:185.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.