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Safety Data Sheet (SDS) Pacific Blue Anti-mouse Ly108 Antibody     Product Data Sheet (PDF)    
Pacific Blue™ Anti-mouse Ly108 Antibody
1273040 100 µg $270.00       
Clone: 330-AJ
Isotype: Mouse IgG2a, κ
Reactivity: Mouse
Immunogen: Thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

C57BL/6 splenocytes stained with 330-AJ

C57BL/6 splenocytes stained with 330-AJ Pacific Blue™ and CD45R/B220 (RA3-6B2) PE

C57BL/6 splenocytes double stained with

C57BL/6 splenocytes double stained with mouse IgG2a isotype control Pacific Blue™ and CD45R/B220 (RA3-6B2) PE



Description:

Mouse Ly108, also known as SLAMF6 and NTB-A (NK cell, T cell, B cell antigen), is one of the members in The Signaling Lymphocytic Activation Molecule (SLAM) family of immune receptors. It is expressed on T cells, B cells, macrophages, dendritic cells, NK cells, and granulocytes. Homophilic interaction of Ly108 is involved in augmenting cytotoxicity of NK cells. Ly108 has been shown to function on NK cells by augmenting cytotoxicity. It was reported that Ly108 plays an important role in CD4+ T cell responses and innate immunity to bacteria and parasites. In a mouse with a targeted disruption of the Ly108 gene, CD4+ T cells and innate responses are defective. SLAM family of receptors has been implicated in the pathophysiology of autoimmunity. For instance, Ly108 is strongly linked to lupus susceptibility in mice. Ly108 may censor self-reactive B cells as a potential regulator of tolerance checkpoints, safeguarding against autoimmunity. Therefore, Ly108 serves as a regulator of both innate and adaptive immune responses.

Other Names: SLAM family receptor, SLAMF6, TCOM, SF2000, KALI, SF-3, NTBA, NTB-A, NK-T-B antigen
Structure: A glycoprotein of the signaling lymphocytic activation molecule family of cell surface receptor. It is a novel member of the CD2 subfamily of the Ig superfamily.
Distribution: Expressed on T cells, B cells, macrophages, dendritic cells, NK cells, and granulocytes.
Function: Play an important role in CD4+ T cell responses, innate immunity to bacteria and parasites, and NK cell function.
Ligand Receptor: Ly108
Antigen References:

1. Howie D, et al. 2005. J. Immunology. 174 (10):5931
2. Kumar KR, et al. 2006. Science 312(5780):1665
3. Zhong MC, et al. 2008. J. Biol. Chem. 283 (28):19255
4. Peck SR et al. 2000. Immunogenetics. 52:63


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.