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Safety Data Sheet (SDS) Purified Anti-mouse CD40 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD40 Antibody
1114010 500 µg $195.00       
Clone: 1C10
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP - Reported in the literature

Application Notes:

The 1C10 antibody has been reported to stimulate B cell proliferation and block CD40-CD40L interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation, blocking5,6 of CD40 ligand binding, and in vitro stimulation of B cell proliferation1-3.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Ferlin WG, et al. 1998. Eur. J. Immunol. 28:525. (FC, Costim)
2. Ferlin WG, et al. 1996. Eur. J. Immunol. 26:2911. (Costim)
3. Heath A, et al. 1994. Eur. J. Immunol. 24:1828. (Costim)
4. Ashman RF, et al. 2005. Int. Immunol. 17:411.
5. Peláez B, et al. 2001. J. Immunol. 166:6608. (Block)
6. Zhang P, et al. 2007. J. Immunol. 178:474. (Block)
7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
8. Ponomarev ED, et al. 2007. J.Immunol.178:39. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with purified 1C10, followed by anti-rat IgG FITC



Description:

CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Other Names: Bp50, TNFRSF5
Structure: TNFR superfamily, 48 kD
Distribution: B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors
Function: Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
Ligand Receptor: CD40L (CD154)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.