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Safety Data Sheet (SDS) Biotin Anti-mouse IgEa Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse IgEa Antibody
2644020 500 µg $210.00       
Clone: UH297
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: BALB/c mouse IgE anti-DNP mAb from DNPε 117 hybridoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

ELISA - Quality tested
FC, WB - Reported in the literature

Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA detection, the suggested use of this reagent is 1-2 µg per ml. It's recommended that the reagent be titrated for optimal performance for each application.
Application References: 1. Usui M, et al. 1993. Immunogenetics. 37:301-304
2. Yamada M, et al. 1994. Immunogenetics. 40:296-299


Description:

The UH297 antibody reacts specifically with mouse soluble immunoglobulin E of Igh-Ca haplotype (e.g., A, AKR, BALB/c, C3H/He, CBA). It does not react with IgE Igh-cb haplotype (e.g., C57BL/6, C57BL/10, SJL) and other Ig isotypes. UH297 mAb can be used for immunofluorescent staining of cell surface IgEa and ELISA applications.

Other Names: 1. Usui, M. et al. 1993. Immunogenetics. 37:301-304
2. Yamada, M. et al. 1994. Immunogenetics. 40:296-299